To explore the causes and roots of cancer development, I compared the differences between cancer tissue and control normal tissue.
Two ways I did this were by comparing cancer and normal tissue by examining differences in
the genome and gene expression profile.
By looking at the genome of a tissue, I examined both the genes and non-coding DNA, and more.
Through this, I examined what a cell’s sequence could possibly do.
By looking at the gene expression profile, I examined the functionality of the genome
(what genes were being transcribed into RNA) and what a cell’s sequence was actually doing at a point in time.
Technology like next-generation high-throughput sequencing (NGS)
facilitated this work and allowed the parallelization of DNA and RNA sequencing to greatly increase the number of sequences
read each run.